Simultaneous electrochemiluminescence detection of anisodamine, atropine, and scopolamine in Flos daturae by capillary electrophoresis using β-Cyclodextrin as additive

This work developed a simple and sensitive method for simultaneous determination of three effective ingredients, atropine, scopolamine and anisodamine, in Flos daturae based on capillary electrophoresis coupled with electro-chemiluminescence detection. beta-Cyclodextrin was used as an additive to the running buffer for obtaining the absolute separation. The proposed method displayed the linear ranges from 0.2 to 100, 0.2 to 100 and 20 to 200 mu M for anisodamine, atropine and scopolamine with correlation coefficients more than 0.99, respectively. This method showed the relative standard deviations less than 4% and 6% for detection of migration time and peak height, respectively, and was suitable for the determination of these tropane alkaloids in plants and valuable in clinical and biochemical laboratories for quality control.