Simultaneous electrochemiluminescence detection of anisodamine, atropine, and scopolamine in Flos daturae by capillary electrophoresis using β-Cyclodextrin as additive

被引：41

作者：

Li, Jianguo

Chun, Yuan

Ju, Huangxian ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, Educ Minist China, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

[2] Suzhou Univ, Sch Chem & Chem Engn, Suzhou 215006, Peoples R China

来源：

ELECTROANALYSIS | 2007年 / 19卷 / 15期

关键词：

capillary electrophoresis; electrochemiluminescence; beta-Cyclodextrin; anisodamine; atropine; scopolamine;

D O I：

10.1002/elan.200703903

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work developed a simple and sensitive method for simultaneous determination of three effective ingredients, atropine, scopolamine and anisodamine, in Flos daturae based on capillary electrophoresis coupled with electro-chemiluminescence detection. beta-Cyclodextrin was used as an additive to the running buffer for obtaining the absolute separation. The proposed method displayed the linear ranges from 0.2 to 100, 0.2 to 100 and 20 to 200 mu M for anisodamine, atropine and scopolamine with correlation coefficients more than 0.99, respectively. This method showed the relative standard deviations less than 4% and 6% for detection of migration time and peak height, respectively, and was suitable for the determination of these tropane alkaloids in plants and valuable in clinical and biochemical laboratories for quality control.

引用

页码：1569 / 1574

页数：6

共 43 条

[31] Enantioseparation of atropine by capillary electrophoresis using sulfated β-cyclodextrin:: application to a plant extract [J].