An assessment of histone-modification antibody quality

被引：287

作者：

Egelhofer, Thea A. ^{[3
]}

Minoda, Aki ^{[4
,5
]}

Klugman, Sarit ^{[6
,7
]}

Lee, Kyungjoon ^{[8
]}

Kolasinska-Zwierz, Paulina ^{[9
,10
]}

Alekseyenko, Artyom A. ^{[11
,12
]}

Cheung, Ming-Sin ^{[9
,10
]}

Day, Daniel S. ^{[8
]}

Gadel, Sarah ^{[13
]}

Gorchakov, Andrey A. ^{[11
,12
]}

Gu, Tingting ^{[13
]}

Kharchenko, Peter V. ^{[8
]}

Kuan, Samantha ^{[6
,7
]}

Latorre, Isabel ^{[9
,10
]}

Linder-Basso, Daniela ^{[14
]}

Luu, Ying ^{[6
,7
]}

Ngo, Queminh ^{[6
,7
]}

Perry, Marc ^{[15
]}

Rechtsteiner, Andreas ^{[3
]}

Riddle, Nicole C. ^{[13
]}

Schwartz, Yuri B. ^{[14
]}

Shanower, Gregory A. ^{[14
]}

Vielle, Anne ^{[9
,10
]}

Ahringer, Julie ^{[9
,10
]}

Elgin, Sarah C. R. ^{[13
]}

Kuroda, Mitzi I. ^{[11
,12
]}

Pirrotta, Vincenzo ^{[14
]}

Ren, Bing ^{[6
,7
]}

Strome, Susan ^{[3
]}

Park, Peter J. ^{[8
]}

Karpen, Gary H. ^{[4
,5
]}

Hawkins, R. David ^{[6
,7
]}

Lieb, Jason D. ^{[1
,2
]}

机构：

[1] Carolina Ctr Genome Sci, Dept Biol, Chapel Hill, NC USA

[2] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA

[3] Univ Calif Santa Cruz, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA

[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[5] Lawrence Berkeley Natl Lab, Dept Genome Dynam, Berkeley, CA USA

[6] Ludwig Inst Canc Res, La Jolla, CA USA

[7] Univ Calif San Diego, San Diego Sch Med, Dept Cellular & Mol Med, La Jolla, CA 92093 USA

[8] Harvard Univ, Sch Med, Ctr Biomed Informat, Boston, MA USA

[9] Univ Cambridge, Gurdon Inst, Cambridge, England

[10] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England

[11] Brigham & Womens Hosp, Dept Med, Div Genet, Boston, MA 02115 USA

[12] Harvard Univ, Sch Med, Dept Genet, Boston, MA USA

[13] Washington Univ, Dept Biol, St Louis, MO 63130 USA

[14] Rutgers State Univ, Dept Mol Biol & Biochem, Piscataway, NJ 08855 USA

[15] Ontario Inst Canc Res, Toronto, ON, Canada

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 01期

基金：

美国国家卫生研究院;

关键词：

GENOME;

D O I：

10.1038/nsmb.1972

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).

引用

页码：91 / +

页数：4

共 13 条

[1] ACETYLATION + METHYLATION OF HISTONES + THEIR POSSIBLE ROLE IN REGULATION OF RNA SYNTHESIS [J].

ALLFREY, VG ;

FAULKNER, R ;

MIRSKY, AE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 51 (05) :786-+

[2] The NIH Roadmap Epigenomics Mapping Consortium [J].

Bernstein, Bradley E. ;

Stamatoyannopoulos, John A. ;

Costello, Joseph F. ;

Ren, Bing ;

Milosavljevic, Aleksandar ;

Meissner, Alexander ;

Kellis, Manolis ;

Marra, Marco A. ;

Beaudet, Arthur L. ;

Ecker, Joseph R. ;

Farnham, Peggy J. ;

Hirst, Martin ;

Lander, Eric S. ;

Mikkelsen, Tarjei S. ;

Thomson, James A. .

NATURE BIOTECHNOLOGY, 2010, 28 (10) :1045-1048

[3] Unlocking the secrets of the genome [J].

Celniker, Susan E. ;

Dillon, Laura A. L. ;

Gerstein, Mark B. ;

Gunsalus, Kristin C. ;

Henikoff, Steven ;

Karpen, Gary H. ;

Kellis, Manolis ;

Lai, Eric C. ;

Lieb, Jason D. ;

MacAlpine, David M. ;

Micklem, Gos ;

Piano, Fabio ;

Snyder, Michael ;

Stein, Lincoln ;

White, Kevin P. ;

Waterston, Robert H. .

NATURE, 2009, 459 (7249) :927-930

[4] Distinct Epigenomic Landscapes of Pluripotent and Lineage-Committed Human Cells [J].

Hawkins, R. David ;

Hon, Gary C. ;

Lee, Leonard K. ;

Ngo, QueMinh ;

Lister, Ryan ;

Pelizzola, Mattia ;

Edsall, Lee E. ;

Kuan, Samantha ;

Luu, Ying ;

Klugman, Sarit ;

Antosiewicz-Bourget, Jessica ;

Ye, Zhen ;

Espinoza, Celso ;

Agarwahl, Saurabh ;

Shen, Li ;

Ruotti, Victor ;

Wang, Wei ;

Stewart, Ron ;

Thomson, James A. ;

Ecker, Joseph R. ;

Ren, Bing .

CELL STEM CELL, 2010, 6 (05) :479-491

[5] The Organization of Histone H3 Modifications as Revealed by a Panel of Specific Monoclonal Antibodies [J].

Kimura, Hiroshi ;

Hayashi-Takanaka, Yoko ;

Goto, Yuji ;

Takizawa, Nanako ;

Nozaki, Naohito .

CELL STRUCTURE AND FUNCTION, 2008, 33 (01) :61-73

[6] Differential chromatin marking of introns and expressed exons by H3K36me3 [J].

Kolasinska-Zwierz, Paulina ;

Down, Thomas ;

Latorre, Isabel ;

Liu, Tao ;

Liu, X. Shirley ;

Ahringer, Julie .

NATURE GENETICS, 2009, 41 (03) :376-381

[7] Chromatin modifications and their function [J].

Kouzarides, Tony .

CELL, 2007, 128 (04) :693-705

[8] ChIP-seq: advantages and challenges of a maturing technology [J].

Park, Peter J. .

NATURE REVIEWS GENETICS, 2009, 10 (10) :669-680

[9]

PAZIN M, 2000, DROSOPHILA PROTOCOLS, P553

[10] The Histone H3K36 Methyltransferase MES-4 Acts Epigenetically to Transmit the Memory of Germline Gene Expression to Progeny [J].

Rechtsteiner, Andreas ;

Ercan, Sevinc ;

Takasaki, Teruaki ;

Phippen, Taryn M. ;

Egelhofer, Thea A. ;

Wang, Wenchao ;

Kimura, Hiroshi ;

Lieb, Jason D. ;

Strome, Susan .

PLOS GENETICS, 2010, 6 (09)

← 1 2 →