Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus:: characterisation and role in organophosphate resistance

被引:91
作者
Baxter, GD [1 ]
Barker, SC
机构
[1] Univ Queensland, Dept Parasitol, Brisbane, Qld 4072, Australia
[2] Univ Queensland, Ctr Mol & Cell Biol, Brisbane, Qld 4072, Australia
关键词
acetylcholinesterase; cattle tick; organophosphate resistance; insecticide; cDNA cloning; alternative splicing;
D O I
10.1016/S0965-1748(98)00034-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Acetylcholinesterase is the target of organophosphate and carbamate pesticides. Organophosphate resistance is widespread in the cattle tick, Boophilus microplus, in Australia. We have isolated a cDNA of acetylcholinesterase from B. microplus and show that it would encode a protein 62 kDa in size. The predicted amino acid sequence contains all the residues characteristic of an acetylcholinesterase. Alternative splicing of the transcript was detected at both the 5' and 3' ends. Alternative splicing at the 5' end would result in two proteins differing by six amino acids. This is the first report of alternative splicing of the N-terminal coding region in a cholinesterase. No point mutations were detected in the acetylcholinesterase gene from organophosphate resistant strains of B. microplus. Alternative explanations for resistance to organophosphates in B. microplus are discussed. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:581 / 589
页数:9
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