Cytogenetic localization of 44 new coding sequences in the horse

被引:22
作者
Godard, S [1 ]
Vaiman, A [1 ]
Schibler, L [1 ]
Mariat, D [1 ]
Vaiman, D [1 ]
Cribiu, EP [1 ]
Guérin, G [1 ]
机构
[1] INRA, Dept Denet Anim, Lab Genet Biochim & Cytogenet, Ctr Rech Jouy, F-78352 Jouy En Josas, France
关键词
D O I
10.1007/s003350010206
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of this study was to increase the number of genes assigned by in situ hybridization to equine chromosomes and thus the number of links for comparative mapping with other species. Forty-four new sequences were added to the horse cytogenetic map by FISH mapping of BAC clones containing genes (35) or ESTs (9). Three approaches were developed: use of horst BAC clones screened with (i) horse EST primers, (ii) interspecific consensus intraexonic primers, and (iii) use of goat BAC containing genes previously localized on goat chromosomes. Present data suggest that the second approach is the most promising. A total of 46 segments containing one or several genes could be compared, among which 40 loci could be included in 16 synteny groups between human and horse, displaying one ordered segment and several breaking points along chromosomes. All single BAC localizations confirm the most recent mapping data. Twenty-six out of 31 chromosomes now contain a gene mapped by in situ hybridization, and 14 new arm-to-arm segment homologies were revealed.
引用
收藏
页码:1093 / 1097
页数:5
相关论文
共 34 条
[11]   A second-generation linkage map of the sheep genome [J].
de Gortari, MJ ;
Freking, BA ;
Cuthbertson, RP ;
Kappes, SM ;
Keele, JW ;
Stone, RT ;
Leymaster, KA ;
Dodds, KG ;
Crawford, AM ;
Beattie, CW .
MAMMALIAN GENOME, 1998, 9 (03) :204-209
[12]   QTL mapping to QTL cloning: Mice to the rescue [J].
Georges, M .
GENOME RESEARCH, 1997, 7 (07) :663-665
[13]   Construction of a horse BAC library and cytogenetical assignment of 20 type I and type II markers [J].
Godard, S ;
Schibler, L ;
Oustry, A ;
Cribiu, EP ;
Guérin, G .
MAMMALIAN GENOME, 1998, 9 (08) :633-637
[14]   Characterization, genetic and physical mapping analysis of 36 horse plasmid and cosmid-derived microsatellites [J].
Godard, S ;
Vaiman, D ;
Oustry, A ;
Nocart, M ;
Bertaud, M ;
Guzylack, S ;
Meriaux, JC ;
Cribiu, EP ;
Guerin, G .
MAMMALIAN GENOME, 1997, 8 (10) :745-750
[15]   A radiation hybrid map of bovine Chromosome 7 and comparative mapping with human Chromosome 19 p arm [J].
Gu, ZX ;
Womack, JE ;
Kirkpatrick, BW .
MAMMALIAN GENOME, 1999, 10 (11) :1112-1114
[16]   Report of the International Equine Gene Mapping Workshop:: male linkage map [J].
Guérin, G ;
Bailey, E ;
Bernoco, D ;
Anderson, I ;
Antczak, DF ;
Bell, K ;
Binns, MM ;
Bowling, AT ;
Brandon, R ;
Cholewinski, G ;
Cothran, EG ;
Ellegren, H ;
Förster, M ;
Godard, S ;
Horin, P ;
Ketchum, M ;
Lindgren, G ;
McPartlan, H ;
Mériaux, JC ;
Mickelson, JR ;
Millon, LV ;
Murray, J ;
Neau, A ;
Roed, K ;
Sandberg, K ;
Shiue, YL ;
Skow, LC ;
Stott, M ;
Swinburne, J ;
Valberg, SJ ;
Van Haeringen, H ;
Van Haeringen, WA ;
Ziegle, J .
ANIMAL GENETICS, 1999, 30 (05) :341-354
[17]   Traced orthologous amplified sequence tags (TOASTs) and mammalian comparative maps [J].
Jiang, Z ;
Priat, C ;
Galibert, F .
MAMMALIAN GENOME, 1998, 9 (07) :577-587
[18]   A second-generation linkage map of the bovine genome [J].
Kappes, SM ;
Keele, JW ;
Stone, RT ;
Sonstegard, TS ;
Smith, TPL ;
McGraw, RA ;
LopezCorrales, NL ;
Beattie, CW .
GENOME RESEARCH, 1997, 7 (03) :235-249
[19]  
Le Provost F, 2000, GENET SEL EVOL, V32, P311, DOI 10.1051/gse:2000121
[20]   Horse v-fes feline sarcoma viral oncogene homologue; pyruvate kinase, muscle type 2; plasminogen; beta spectrin, non-erythrocytic 1; thymidylate synthetase; and microsatellite LEX078 map to 1q14-q15, 1q21, 31q12-q14, 15q22, 8q12-q14, and 14q27, respectively [J].
Lear, TL ;
Piumi, F ;
Terry, R ;
Guerin, G ;
Bailey, E .
CHROMOSOME RESEARCH, 2000, 8 (04) :361-361