Simple thin layer chromatography method with fibre optic remote sensor for fluorimetric quantification of tryptophan and related metabolites

被引：11

作者：

Aponte, RL

Diaz, JA

Perera, AA

Diaz, VG

机构：

[1] Department of Analytical Chemistry, Food Science and Toxicology, University of La Laguna

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1996年 / 19卷 / 05期

关键词：

D O I：

10.1080/10826079608005530

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tryptophan (TP), 5-hydroxytryptophan (5-HTP), 3-indoleacetic acid (IAA) and serotonin (5-HT) were separated by TLC, with chloroform-methanol-ammonia (12-7-1) (v-v-v) as eluent and cellulose as stationary phase. A fibre optic-based fluorescence instrument for in situ scanning was used for quantitative measurements. The compounds were determined over the range 10-100 ng, with relative standard deviations between 1.70-6.52% and detection limits over the range 16.39-22.50 ng.

引用

收藏

页码：687 / 698

页数：12

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