Different regioselectivity of two commercial acetylesterases for hydrolysis of paracetylated maltose

被引：2

作者：

Copinet, A ^{[1
]}

Couturier, Y ^{[1
]}

Prudhomme, JC ^{[1
]}

机构：

[1] Grepac Esiec, F-51086 Reims 2, France

来源：

BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 05期

关键词：

D O I：

10.1023/A:1005427926694

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two commercial acetylesterases from Aspergillus niger with an associated amyloglucosidase degraded 6% and 25% of peracetylated maltose. This difference is explained by the regioselectivity of deacetylation :the acetylesterase from Sigma attacked the C1 and C2 atoms of the glucosidic ring whereas acetylesterase from Viscozyme attacked the C1 and C6 atoms and cleaved the bond alpha 1-->4 of the substrate.

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页码：447 / 450

页数：4

相关论文

共 9 条

[1] REGIOSELECTIVITY ENHANCEMENT BY PARTIAL-PURIFICATION OF LIPASE FROM ASPERGILLUS-NIGER [J].

CHEN, HP ;

HSIAO, KF ;

WU, SH ;

WANG, KT .

BIOTECHNOLOGY LETTERS, 1995, 17 (03) :305-308

[2] BIOFRAGMENTATION OF ACETYLATED STARCH BY THE ALPHA-AMYLASE OF ASPERGILLUS-ORYZAE [J].

COMA, V ;

COPINET, A ;

COUTURIER, Y ;

PRUDHOMME, JC .

STARCH-STARKE, 1995, 47 (03) :100-107

[3] Hydrolysis of acetylated starches with a thermostable alpha-amylase [J].

Copinet, A ;

Coma, V ;

Legin, E ;

Couturier, Y ;

Prudhomme, JC .

STARCH-STARKE, 1997, 49 (12) :492-498

[4] THE CLASSIFICATION, PREPARATION, AND UTILITY OF DEGRADABLE POLYMERS [J].

HOCKING, PJ .

JOURNAL OF MACROMOLECULAR SCIENCE-REVIEWS IN MACROMOLECULAR CHEMISTRY AND PHYSICS, 1992, C32 (01) :35-54

[5] Proton NMR spectroscopy assignment of D-glucose residues in highly acetylated starch [J].

Laignel, B ;

Bliard, C ;

Massiot, G ;

Nuzillard, JM .

CARBOHYDRATE RESEARCH, 1997, 298 (04) :251-260

[6]

Leatherbarrow R. J., 1987, ENZFITTER NONLINEAR

[7]

ONTENIENTE JP, 1996, MAT TECHNIQUES, V3, P31

[8] THE ACETYLATION AND ENZYMATIC DEGRADATION OF STARCH FILMS [J].

PARANDOOSH, S ;

HUDSON, SM .

JOURNAL OF APPLIED POLYMER SCIENCE, 1993, 48 (05) :787-791

[9] Effects of substitution site on acetyl amylose biodegradability by amylase enzymes [J].

Roesser, DS ;

McCarthy, SP ;

Gross, RA ;

Kaplan, DL .

MACROMOLECULES, 1996, 29 (01) :1-9