Local stoichiometries determined by counting individual molecules

被引：82

作者：

Schmidt, T

Schutz, GJ

Gruber, HJ

Schindler, H

机构：

[1] Institute for Biophysics, University of Linz, 4040 Linz

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 24期

关键词：

D O I：

10.1021/ac960710g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The capability of optical single-molecule microscopy for quantitative determination of local stoichiometries is demonstrated. Biotin-streptavidin embedded in a phospholipid membrane was chosen as a model system. At a local resolution of 30 mn and a time resolution of 35 ms, it was possible to determine local stoichiometries between one and four occurring in this system, The ''stoichiometric resolution'' of the method is analyzed by statistical means. From this a quantitative criterion is derived for the reliability in assigning a defined number of fluorophores to each individual fluorescence peak observed. It is shown that only a few (n approximate to 10) observations are sufficient for reliable assignments.

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收藏

页码：4397 / 4401

页数：5

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