Development of a novel protein microarray method for Serotyping Salmonella enterica strains

被引:38
作者
Cai, HY
Lu, L
Muckle, CA
Prescott, JF
Chen, S
机构
[1] Univ Guelph, Lab Serv, Guelph, ON N1H 8J7, Canada
[2] Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, OIE Reference Lab Salmonellosis, Guelph, ON, Canada
[3] Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada
关键词
D O I
10.1128/JCM.43.7.3427-3430.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An antibody microarray assay was developed for Salmonella serotyping based on the Kauffmann-White scheme. A model (8 by 15) array was constructed using 35 antibodies for identification of 20 common Salmonella serovars and evaluated using 117 target and 73 nontarget Salmonella strains. The assay allowed complete serovar identification of 86 target strains and partial identification of 30 target strains and allowed exclusion of the 73 nontarget strains from the target serovars.
引用
收藏
页码:3427 / 3430
页数:4
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