Differential depletion of human respiratory tract antioxidants in response to ozone challenge

被引:35
作者
Mudway, IS
Housley, D
Eccles, R
Richards, RJ
Datta, AK
Tetley, TD
Kelly, FJ
机构
[1] UNIV WALES COLL CARDIFF,SCH MOL & MED BIOSCI,CARDIFF CF1 3US,S GLAM,WALES
[2] ST THOMAS HOSP,RAYNE INST,LONDON SE1 7EH,ENGLAND
[3] CHARING CROSS & WESTMINSTER MED SCH,DEPT MED,LONDON W6 8RF,ENGLAND
基金
英国医学研究理事会;
关键词
ozone; antioxidant; epithelial lining fluid; lung; uric acid; ascorbic acid; glutathione;
D O I
10.3109/10715769609149072
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The toxicity of ozone, the major component of photochemical smog, is related to its powerful oxidising ability, and many of its deleterious effects are mediated through free radical reactions. As the majority of ozone oxidation events are thought to be confined to the pulmonary epithelial lining fluid, we studied the interaction of ozone with a range of small molecular weight antioxidants found within this compartment: ascorbic acid (AH(2)), uric acid (UA), and reduced glutathione (GSH). Epithelial lining fluid obtained as bronchoalveolar lavage (BAL) fluid, was taken from 16 male subjects and the antioxidant concentrations determined for each subject. BAL fluid samples from nine of these subjects were then exposed, using an interfacial exposure system, to a range (50-1000 ppb) of ozone concentrations. Both AH(2) and UA were consumed by ozone in a time and ozone concentration dependent manner, with mean consumption rates of 1.7 +/- 0.8 and 1.0 +/- 0.5 pmol L(-1) s(-1) ppb(-1), respectively. Considerable intersubject variation was however observed. The individual rates of consumption for each antioxidant were significantly correlated with the respective initial antioxidant concentration. In contrast, although GSH was consumed at 50 ppb ozone, the rate of consumption did not change with increasing ozone concentration. We conclude that there is differential depletion of BAL fluid antioxidants, suggesting a reactivity hierarchy toward ozone in human ELF of AH(2) > UA much greater than GSH.
引用
收藏
页码:499 / 513
页数:15
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