Co-immobilized Lignin Peroxidase and Manganese Peroxidase From Coriolus Versicolor Capable of Decolorizing Molasses Waste Water

被引：10

作者：

Ran, Yanhong ^{[1
]}

Che, Zhifei ^{[1
]}

Chen, Wanqun ^{[2
]}

机构：

[1] Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China

[2] Jinan Univ, Med Coll, Guangzhou 510632, Guangdong, Peoples R China

来源：

APPLIED MECHANICS AND MECHANICAL ENGINEERING II, PTS 1 AND 2 | 2012年 / 138-139卷

关键词：

Lignin peroxidase; manganese peroxidase; chitosan microsphere; co-immobilization; BJERKANDERA SP BOS55; GENE DELIVERY; LACCASE; TRANSFECTION; COMPLEXES; MECHANISM;

D O I：

10.4028/www.scientific.net/AMM.138-139.1067

中图分类号：

TH [机械、仪表工业];

学科分类号：

0802 ;

摘要：

This paper explains how the Lignin peroxidase and Manganese Peroxidase from Coriolus Versicolor were co-immobilized by chitosan microspheres. It studies kinetic character of the enzyme after co-immobilization. Optimum Lip and MnP activity obtained at 30-35 degrees C for 14 hours in pH 8.4 glutaraldehyde solutions during immobilized to chitosan microspheres which prepared by coagulation in NaOH: methanol=3:2. When kept at 50 degrees C for 6h, more than 80% of the immobilized enzyme activity remained, while the free enzymes were inactive under the same conditions. The co-immobilized enzyme can remain 70% activity after two weeks while both of the free enzymes inactive. Compared with the free enzymes, temperature and time stability of the co-immobilized enzyme was considerably improved.

引用

页码：1067 / +

页数：2