Rosmarinus officinalis Essential Oil Improves Scopolamine-Induced Neurobehavioral Changes via Restoration of Cholinergic Function and Brain Antioxidant Status in Zebrafish (Danio rerio)

被引：48

作者：

Capatina, Luminita ^{[1
]}

Boiangiu, Razvan Stefan ^{[1
]}

Dumitru, Gabriela ^{[1
]}

Napoli, Edoardo Marco ^{[2
]}

Ruberto, Giuseppe ^{[2
]}

Hritcu, Lucian ^{[1
]}

Todirascu-Ciornea, Elena ^{[1
]}

机构：

[1] Alexandru Ioan Cuza Univ, Fac Biol, Dept Biol, Iasi 700506, Romania

[2] Natl Res Council ICB CNR, Inst Biomol Chem, I-95126 Catania, Italy

来源：

ANTIOXIDANTS | 2020年 / 9卷 / 01期

关键词：

Rosmarinus officinalis; essential oil; scopolamine; anxiety; memory; oxidative stress; INDUCED MEMORY IMPAIRMENT; DONEPEZIL TREATMENT; L; EXTRACT; AVOIDANCE; DEFICITS;

D O I：

10.3390/antiox9010062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Rosmarinus officinalis L. is a traditional herb with various therapeutic applications such as antibacterial, antioxidant, anti-inflammatory, antidepressant, and anticholinesterase activities, and can be used for the prevention or treatment of dementia. In the present study, we tested whether Rosmarinus officinalis L. could counteract scopolamine-induced anxiety, dementia, and brain oxidative stress in the zebrafish model and tried to find the underlying mechanism. Rosmarinus officinalis L. essential oil (REO: 25, 150, and 300 mu L/L) was administered by immersion to zebrafish (Danio rerio) once daily for eight days while scopolamine (100 mu M) treatment was delivered 30 min before behavioral tests. The antidepressant and cognitive-enhancing actions of the essential oil in the scopolamine zebrafish model was measured in the novel tank diving test (NTT) and Y-maze test. The chemical composition was identified by Gas chromatograph-Mass spectrometry (GC-MS) analysis. The brain oxidative status and acetylcholinesterase (AChE) activity was also determined. REO reversed scopolamine-induced anxiety, memory impairment, and brain oxidative stress. In addition, a reduced brain AChE activity following the administration of REO in scopolamine-treated fish was observed. In conclusion, REO exerted antidepressant-like effect and cognitive-enhancing action and was able to abolish AChE alteration and brain oxidative stress induced by scopolamine.

引用

页数：14

共 40 条

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