Antioxidant enzymatic defences in human follicular fluid: characterization and age-dependent changes

被引:167
作者
Carbone, MC
Tatone, C
Delle Monache, S
Marci, R
Caserta, D
Colonna, R
Amicarelli, F
机构
[1] Univ Aquila, Dept Basic & Appl Biol, I-67100 Laquila, Italy
[2] Univ Aquila, Dept Biomed Sci & Technol, I-67100 Laquila, Italy
[3] Univ Aquila, Dept Expt Med, I-67100 Laquila, Italy
[4] Univ Aquila, Ctr Assisted Reprod, I-67100 Laquila, Italy
关键词
ageing; antioxidant enzymes; follicular fluid; oxidative stress; reproductive potential;
D O I
10.1093/molehr/gag090
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The aim of this work was to study the antioxidant enzymatic defences in human follicular fluid and investigate their possible changes during reproductive ageing. To this end, we tested the specific activities and protein expression of enzymes involved in reactive oxygen species (ROS) scavenging and in detoxification of ROS byproducts in follicular fluid from young (range 27-32 years, n = 12) and older (range 39-45 years, n = 12) women participating in an IVF programme. Results show that all the tested enzymes [superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione transferase, glutathione reductase] were significantly expressed in human follicular fluid. However, when the two age groups were compared, we found that follicular fluid from older women exhibited a reduced level of glutathione transferase and catalase activities and a higher level of SOD activity. Immunoblot analysis revealed that ageing was associated with decreased protein expression of GST Pi isoform and did not affect SOD and catalase protein expression. Taken together, these findings indicate that reproductive ageing is accompanied by a change in the antioxidant enzymatic pattern that could impair ROS scavenging efficiency in the follicular environment.
引用
收藏
页码:639 / 643
页数:5
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