Parametric optimization and biochemical regulation of extracellular tannase from Aspergillus japonicus

被引：78

作者：

Bradoo, S ^{[1
]}

Gupta, R ^{[1
]}

Saxena, RK ^{[1
]}

机构：

[1] UNIV DELHI, DEPT MICROBIOL, NEW DELHI 110021, INDIA

来源：

PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 02期

关键词：

D O I：

10.1016/S0032-9592(96)00056-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aspergillus japonicus produces maximum extracellular tannase activity after 24 h incubation. The enzyme is produced constitutively on simple and complex sugar substrates but activity is doubled in the presence of tannic acid as sole carbon source. Parametric optimization of the enzyme yielded 1.13-fold increase in enzyme production (33.06 U/ml) at 30 degrees C and pH 6.6 with 0.2% glucose and 2% tannic acid in Czapek-Dox's minimal medium. The enzyme shows strong end-product repression with gallic acid. Copyright (C) 1996 Elsevier Science Ltd

引用

页码：135 / 139

页数：5

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