Heterochromia in designed metallopeptides:: Geometry-selective binding of CdII in a de novo peptide

被引：36

作者：

Iranzo, Olga

Cabello, Chris

Pecoraro, Vincent L. ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Biophys Res Div, Ann Arbor, MI 48109 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 35期

关键词：

cadmium; coordination modes; de novo peptides; metalloproteins; protein design;

D O I：

10.1002/anie.200701729

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Molecular recognition and control of the physical properties of metal ions are major challenges in metalloprotein design. The newly designed peptide Grand L16PenL26AL30C binds two CdII ions, each with a different coordination geometry - trigonal planar or pseudotetrahedral (see picture; Cd purple, S yellow, O blue). The physical properties of the two centers, such as site selectivity and pH dependence of binding, differ as well. (Graph Presented) © 2007 Wiley-VCH Verlag GmbH &. Co. KGaA.

引用

收藏

页码：6688 / 6691

页数：4

相关论文

共 18 条

[1]

Chan W., 1999, Fmoc solid phase peptide synthesis: a practical approach

[2]

COBAS C, 2000, MESTREC VERSION 2 3

[3] X-ray structure of 5-aminolaevulinate dehydratase, a hybrid aldolase [J].

Erskine, PT ;

Senior, N ;

Awan, S ;

Lambert, R ;

Lewis, G ;

Tickle, LJ ;

Sarwar, M ;

Spencer, P ;

Thomas, P ;

Warren, MJ ;

ShoolinginJordan, PM ;

Wood, SP ;

Cooper, JB .

NATURE STRUCTURAL BIOLOGY, 1997, 4 (12) :1025-1031

[4] Thermodynamic model for the stabilization of trigonal thiolato mercury(II) in designed three-stranded coiled coils [J].

Farrer, BT ;

Harris, NP ;

Balchus, KE ;

Pecoraro, VL .

BIOCHEMISTRY, 2001, 40 (48) :14696-14705

[5] Linear free-energy analysis of mercury(II) and cadmium(II) binding to three-stranded coiled coils [J].

Ghosh, D ;

Lee, KH ;

Demeler, B ;

Pecoraro, VL .

BIOCHEMISTRY, 2005, 44 (31) :10732-10740

[6] Assessing the integrity of designed homomeric parallel three-stranded coiled coils in the presence of metal ions [J].

Iranzo, Olga ;

Ghosh, Debdip ;

Pecoraro, Vincent L. .

INORGANIC CHEMISTRY, 2006, 45 (25) :9959-9973

[7] The molecular mechanism of lead inhibition of human porphobilinogen synthase [J].

Jaffe, EK ;

Martins, J ;

Li, J ;

Kervinen, J ;

Dunbrack, RL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (02) :1531-1537

[8]

Lee K.-H., 2006, ANGEW CHEM, V118, P2930

[9] Using nonnatural amino acids to control metal-coordination number in three-stranded coiled coils [J].

Lee, Kyung-Hoon ;

Cabello, Chris ;

Hemmingsen, Lars ;

Marsh, E. Neil G. ;

Pecoraro, Vincent L. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (18) :2864-2868

[10] Retrostructural analysis of metalloproteins: Application to the design of a minimal model for diiron proteins [J].

Lombardi, A ;

Summa, CM ;

Geremia, S ;

Randaccio, L ;

Pavone, V ;

DeGrado, WF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6298-6305