Myosin cleft movement and its coupling to actomyosin dissociation

被引:75
作者
Conibear, PB
Bagshaw, CR [1 ]
Fajer, PG
Kovács, M
Málnási-Czizmadia, A
机构
[1] Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England
[2] Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32310 USA
[3] Eotvos Lorand Univ, Dept Biochem, H-1117 Budapest, Hungary
基金
英国惠康基金; 美国国家科学基金会; 英国生物技术与生命科学研究理事会;
关键词
D O I
10.1038/nsb986
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has long been known that binding of actin and binding of nucleotides to myosin are antagonistic, an observation that led to the biochemical basis for the crossbridge cycle of muscle contraction. Thus ATP binding to actomyosin causes actin dissociation, whereas actin binding to the myosin accelerates ADP and phosphate release. Structural studies have indicated that communication between the actin- and nucleotide-binding sites involves the opening and closing of the cleft between the upper and lower 50K domains of the myosin head. Here we test the proposal that the cleft responds to actin and nucleotide binding in a reciprocal manner and show that cleft movement is coupled to actin binding and dissociation. We monitored cleft movement using pyrene excimer fluorescence from probes engineered across the cleft.
引用
收藏
页码:831 / 835
页数:5
相关论文
共 28 条
  • [21] 3-DIMENSIONAL STRUCTURE OF MYOSIN SUBFRAGMENT-1 - A MOLECULAR MOTOR
    RAYMENT, I
    RYPNIEWSKI, WR
    SCHMIDTBASE, K
    SMITH, R
    TOMCHICK, DR
    BENNING, MM
    WINKELMANN, DA
    WESENBERG, G
    HOLDEN, HM
    [J]. SCIENCE, 1993, 261 (5117) : 50 - 58
  • [22] A structural model for actin-induced nucleotide release in myosin
    Reubold, TF
    Eschenburg, S
    Becker, A
    Kull, FJ
    Manstein, DJ
    [J]. NATURE STRUCTURAL BIOLOGY, 2003, 10 (10) : 826 - 830
  • [23] SARKAR G, 1990, BIOTECHNIQUES, V8, P404
  • [24] A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin
    Shih, WM
    Gryczynski, Z
    Lakowicz, JR
    Spudich, JA
    [J]. CELL, 2000, 102 (05) : 683 - 694
  • [25] TAYLOR EW, 1991, J BIOL CHEM, V266, P294
  • [26] Volkmann N, 2000, NAT STRUCT BIOL, V7, P1147
  • [27] Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes
    Wakelin, S
    Conibear, PB
    Woolley, RJ
    Floyd, DN
    Bagshaw, CR
    Kovács, M
    Málnási-Csizmadia, A
    [J]. JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 2002, 23 (7-8) : 673 - 683
  • [28] Actin-induced closure of the actin-binding cleft of smooth muscle myosin
    Yengo, CM
    De la Cruz, EM
    Chrin, LR
    Gaffney, DP
    Berger, CL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (27) : 24114 - 24119