Genome organization and reverse genetic analysis of a type 1 feline coronavirus

被引：50

作者：

Tekes, Gergely ^{[2
]}

Hofmann-Lehmann, Regina ^{[3
]}

Stallkamp, Iris ^{[2
]}

Thiel, Volker ^{[1
]}

Thiel, Heinz-Juergen ^{[2
]}

机构：

[1] Kantonsspital, Res Dept, CH-9007 St Gallen, Switzerland

[2] Univ Giessen, Inst Virol, D-6300 Giessen, Germany

[3] Univ Zurich, Vetsuisse Fac, Clin Lab, Zurich, Switzerland

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 04期

关键词：

D O I：

10.1128/JVI.02339-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant FCoV (recFCoV) that was shown to bear the same features in vitro as the parental FCoV. Using this system, accessory 3abc genes in the FCoV genome were replaced by green fluorescent protein (recFCoV-GFP) and Renilla luciferase genes (recFCoV-RL). In addition, we showed that feline CD14(+) blood monocytes and dendritic cells can be easily detected after infection with recFCoV-GFP. Thus, our established reverse genetic system provides a suitable tool to study the molecular biology of serotype I FCoV.

引用

页码：1851 / 1859

页数：9

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