Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana

被引：280

作者：

Van Leene, Jelle ^{[1
,2
]}

Hollunder, Jens ^{[1
,2
]}

Eeckhout, Dominique ^{[1
,2
]}

Persiau, Geert ^{[1
,2
]}

Van De Slijke, Eveline ^{[1
,2
]}

Stals, Hilde ^{[1
,2
]}

Van Isterdael, Gert ^{[1
,2
]}

Verkest, Aurine ^{[1
,2
]}

Neirynck, Sandy ^{[1
,2
]}

Buffel, Yelle ^{[1
,2
]}

De Bodt, Stefanie ^{[1
,2
]}

Maere, Steven ^{[1
,2
]}

Laukens, Kris ^{[3
]}

Pharazyn, Anne ^{[4
]}

Ferreira, Paulo C. G. ^{[5
]}

Eloy, Nubia ^{[1
,2
,5
]}

Renne, Charlotte ^{[6
]}

Meyer, Christian ^{[6
]}

Faure, Jean-Denis ^{[6
]}

Steinbrenner, Jens ^{[7
]}

Beynon, Jim ^{[7
]}

Larkin, John C. ^{[8
]}

Van de Peer, Yves ^{[1
,2
]}

Hilson, Pierre ^{[1
,2
]}

Kuiper, Martin ^{[1
,2
]}

De Veylder, Lieven ^{[1
,2
]}

Van Onckelen, Harry ^{[4
]}

Inze, Dirk ^{[1
,2
]}

Witters, Erwin ^{[4
,9
]}

De Jaeger, Geert ^{[1
,2
]}

机构：

[1] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Plant Biotechnol & Genet, B-9000 Ghent, Belgium

[3] Univ Antwerp, Dept Math & Comp Sci, B-2020 Antwerp, Belgium

[4] Univ Antwerp, Ctr Proteome Anal & Mass Spectrometry, Dept Biol, B-2020 Antwerp, Belgium

[5] Univ Fed Rio de Janeiro, Inst Bioquim Med, Ctr Ciencias Saude, Rio De Janeiro, Brazil

[6] INRA AgroParisTech, Inst Jean Pierre Bourgin, Versailles, France

[7] Univ Warwick, Sch Life Sci, Warwick, England

[8] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA

[9] Flemish Inst Technol Res VITO, Mol, Belgium

来源：

MOLECULAR SYSTEMS BIOLOGY | 2010年 / 6卷

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会;

关键词：

Arabidopsis thaliana; cell cycle; interactome; protein complex; protein interactions; TANDEM AFFINITY PURIFICATION; PROTEIN-PROTEIN INTERACTIONS; ANAPHASE-PROMOTING COMPLEX; PLANT-SYSTEMS BIOLOGY; GENOME-WIDE ANALYSIS; DEPENDENT KINASE; DNA-REPLICATION; GENE ONTOLOGY; IDENTIFICATION; EXPRESSION;

D O I：

10.1038/msb.2010.53

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up-and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in-depth biological interpretation demonstrated the hypothesis-generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin-dependent kinase (CDK)-cyclin complexes in plants. For the first time, inhibitory proteins of plant-specific B-type CDKs were discovered and the anaphase-promoting complex was characterized and extended. Important conclusions were that mitotic A-and B-type cyclins form complexes with the plant-specific B-type CDKs and not with CDKA; 1, and that D-type cyclins and S-phase-specific A-type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK-cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants. Molecular Systems Biology 6: 397; published online 10 August 2010; doi:10.1038/msb.2010.53 Subject Categories: plant biology; metabolic & regulatory networks

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