A molecular signature of an arrest of descent in human parturition

被引：32

作者：

Mittal, Pooja ^{[1
,2
,3
]}

Romero, Roberto ^{[1
,2
,4
]}

Tarca, Adi L. ^{[1
,2
,4
]}

Draghici, Sorin ^{[1
,2
]}

Nhan-Chang, Chia-Ling ^{[1
,2
,3
]}

Chaiworapongsa, Tinnakorn ^{[1
,2
,3
]}

Hotra, John ^{[1
,2
]}

Gomez, Ricardo ^{[5
]}

Kusanovic, Juan Pedro ^{[1
,2
,3
]}

Lee, Deug-Chan ^{[1
,2
]}

Kim, Chong Jai ^{[1
,2
]}

Hassan, Sonia S. ^{[1
,2
,3
]}

机构：

[1] NICHHD, Perinatol Res Branch, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA

[2] NICHHD, Perinatol Res Branch, NIH, Dept Hlth & Human Serv, Detroit, MI USA

[3] Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Detroit, MI 48201 USA

[4] Wayne State Univ, Sch Med, Ctr Mol Med & Genet, Detroit, MI USA

[5] Univ Catolica Chile, Sotero Rio Hosp, Ctr Perinatal Diag & Res, Dept Obstet & Gynecol, Puente Alto, Chile

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2011年 / 204卷 / 02期

基金：

美国国家卫生研究院;

关键词：

adenosine triphosphatase; Na plus /K plus transporting; alpha; 1; polypeptide; ATP1A1; COX2; HIF1A; hypoxia inducible factor-1; inflammation; interleukin-6; myometrium; parturition; pregnancy; prostaglandin-endoperoxide synthase 2; PTGS2; spontaneous term labor; systems biology; transcriptomics; TUMOR-NECROSIS-FACTOR; SMOOTH-MUSCLE-CELLS; HUMAN-FETAL MEMBRANES; HUMAN MYOMETRIAL CELLS; CORTICOTROPIN-RELEASING HORMONE; MESSENGER-RNA EXPRESSION; AMNIOTIC-FLUID INTERLEUKIN-6; SPONTANEOUS PRETERM LABOR; PROSTAGLANDIN-E SYNTHASE; HUMAN UTERINE MYOCYTES;

D O I：

10.1016/j.ajog.2010.09.025

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

OBJECTIVE: This study was undertaken to identify the molecular basis of an arrest of descent. STUDY DESIGN: Human myometrium was obtained from women in term labor (TL; n = 29) and arrest of descent (AODes; n = 21). Gene expression was characterized using Illumina HumanHT-12 microarrays. A moderated Student t test and false discovery rate adjustment were applied for analysis. Confirmatory quantitative reverse transcription-polymerase chain reaction and immunoblot were performed in an independent sample set. RESULTS: Four hundred genes were differentially expressed between women with an AODes compared with those with TL. Gene Ontology analysis indicated enrichment of biological processes and molecular functions related to inflammation and muscle function. Impacted pathways included inflammation and the actin cytoskeleton. Overexpression of hypoxia inducible factor-1a, interleukin -6, and prostaglandin-endoperoxide synthase 2 in AODes was confirmed. CONCLUSION: We have identified a stereotypic pattern of gene expression in the myometrium of women with an arrest of descent. This represents the first study examining the molecular basis of an arrest of descent using a genome-wide approach.

引用

页码：177.e15 / 177.e33

页数：19

共 308 条

[81] Monocyte chemotactic protein-1 expression is increased in human gestational tissues during term and preterm labor [J].