Vector systems for the delivery of small interfering RNAs: Managing the RISC

Although the RNAi field is in its infancy, there have been rapid advances in the vector delivery of small interfering RNAs to cells, tissues, and entire organisms. Success has been achieved through a variety of distinct approaches; however there are opportunities to further refine these technologies to more reliably elicit potent and specific RNAi. Pivotal to the continuing evolution of these revolutionary reverse genetic tools will be the attainment of a better understanding of the interplay between vector reagents and the elaborate cellular machinery of the RNAi pathway.