Photodynamic DNA damage mediated by δ-aminolevulinic acid-induced porphyrins

被引：27

作者：

Duez, P ^{[1
]}

Hanocq, M ^{[1
]}

Dubois, J ^{[1
]}

机构：

[1] Free Univ Brussels, Inst Pharm, Serv Chim Bioanalyt Toxicol & Chim Phys Appl, B-1050 Brussels, Belgium

来源：

CARCINOGENESIS | 2001年 / 22卷 / 05期

关键词：

D O I：

10.1093/carcin/22.5.771

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The mutagenic properties of UVA are thought to be predominantly radical-mediated, which supposes endogenous sensitizers. In order to investigate a possible role of porphyrins, their synthesis was induced in a murine leukemia P388D1 cell model by treatment with delta -amino-levulinic acid (delta -ala), Intra-cellular protoporphyrin IX reached a plateau after about 2 h, whereas soluble porphyrins, probably the photostable uro- and/or coproporphyrins, were excreted. Irradiation of treated cells by UVA (tanning lamp) but also by visible light was found to generate in DNA a significant increase of 8-oxo-7,8-dihydro-2'-deoxyguanosine, a mutagenic marker of oxidative damage. The different parameters involved in this photodynamic effect are reported, namely delta -ala concentration and loading time, light dosage and the influence of intracellular and medium-excreted porphyrins, These results point to an implication of porphyrins in solar-induced carcinogenicity but also in possible adverse effects of the medical applications of photodynamic therapy and diagnosis.

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页码：771 / 778

页数：8

相关论文

共 83 条

[61] VISIBLE-LIGHT GENERATES OXIDATIVE DNA-BASE MODIFICATIONS IN HIGH EXCESS OF STRAND BREAKS IN MAMMALIAN-CELLS [J].

PFLAUM, M ;

BOITEUX, S ;

EPE, B .

CARCINOGENESIS, 1994, 15 (02) :297-300

[62] Oxidative DNA damage induced by visible light in mammalian cells: extent, inhibition by antioxidants and genotoxic effects [J].

Pflaum, M ;

Kielbassa, C ;

Garmyn, M ;

Epe, B .

MUTATION RESEARCH-DNA REPAIR, 1998, 408 (02) :137-146

[63] PORPHYRIN PRODUCTION AND EXCRETION BY LONG-TERM CULTURES OF ADULT-RAT HEPATOCYTES AND EFFECT OF LEAD-EXPOSURE [J].

QUINTANILLAVEGA, B ;

HERNANDEZ, A ;

LOPEZ, ML ;

GARCIAVARGAS, G ;

CEBRIAN, ME ;

MENDOZAFIGUEROA, T .

TOXICOLOGY, 1995, 102 (03) :275-283

[64] DNA LESIONS AND DNA-DEGRADATION IN MOUSE LYMPHOMA-L5178Y CELLS AFTER PHOTODYNAMIC TREATMENT SENSITIZED BY CHLOROALUMINUM PHTHALOCYANINE [J].

RAMAKRISHNAN, N ;

OLEINICK, NL ;

CLAY, ME ;

HORNG, MF ;

ANTUNEZ, AR ;

EVANS, HH .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1989, 50 (03) :373-378

[65] Porphyrin sensitization and intracellular calcium changes in the prokaryote Propionibacterium acnes [J].

Ramstad, S ;

Futsaether, CM ;

Johnsson, A .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1997, 40 (02) :141-148

[66] NORMAL OXIDATIVE DAMAGE TO MITOCHONDRIAL AND NUCLEAR-DNA IS EXTENSIVE [J].

RICHTER, C ;

PARK, JW ;

AMES, BN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6465-6467

[67] 8-oxodeoxyguanosine formation in the DNA of cultured cells after exposure to H2O2 alone or with UVB or UVA irradiation [J].

Rosen, JE ;

Prahalad, AK ;

Williams, GM .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1996, 64 (01) :117-122

[68] THE MECHANISM OF BILIRUBIN-PHOTOSENSITIZED DNA STRAND BREAKAGE IN HUMAN-CELLS EXPOSED TO PHOTOTHERAPY LIGHT [J].

ROSENSTEIN, BS ;

DUCORE, JM ;

CUMMINGS, SW .

MUTATION RESEARCH, 1983, 112 (06) :397-406

[69] MISCODING DURING DNA-SYNTHESIS ON DAMAGED DNA TEMPLATES CATALYZED BY MAMMALIAN-CELL EXTRACTS [J].

SHIBUTANI, S ;

GROLLMAN, AP .

CANCER LETTERS, 1994, 83 (1-2) :315-322

[70] SINGLET OXYGEN INDUCED DNA DAMAGE [J].

SIES, H ;

MENCK, CFM .

MUTATION RESEARCH, 1992, 275 (3-6) :367-375

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