Saccharomyces cerevisiae ACR2 gene encodes an arsenate reductase

被引：95

作者：

Mukhopadhyay, R ^{[1
]}

Rosen, BP ^{[1
]}

机构：

[1] Wayne State Univ, Sch Med, Dept Biochem & Mol Biol, Detroit, MI 48201 USA

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 168卷 / 01期

关键词：

yeast; arsenate resistance;

D O I：

10.1016/S0378-1097(98)00430-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ACR2 gene of Saccharomyces cerevisiae was disrupted by insertion of a HIS3 gene. Cells with the disruption were sensitive to arsenate. This phenotype could be complemented by ACR2 on a plasmid. The ACR2 gene was cloned and expressed in Escherichia coli as a malE gene fusion with a C-terminal histidine tag. The combination of chimeric MBP-Acr2-6H protein and yeast cytosol from an ACR2-disrupted strain exhibited arsenate reductase activity. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：127 / 136

页数：10

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