Population dynamics and antagonistic potential of enterococci colonizing the phyllosphere of grasses

被引:43
作者
Ott, EM
Müller, T
Müller, M
Franz, CMAP
Ulrich, A
Gabel, M
Seyfarth, W
机构
[1] Ctr Agr Landscape & Land Use Res Muncheberg, Karlsruhe, Germany
[2] Fed Res Ctr Nutr, Karlsruhe, Germany
关键词
D O I
10.1046/j.1365-2672.2001.01334.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To investigate the spatial and temporal dynamics of enterococci colonizing forage grass and their ability to produce bacteriocins. Methods and Results: Enterococci could be detected on above-ground plant parts throughout the growing season, with high continuity but low cell numbers (2.60 x 10(1)-6.16 x 10(4) cfu g(-1) fresh matter). A total of 750 strains were isolated and identified by their whole-cell protein patterns as Enterococcus faecalis (7.9%), Ent. mundtii (7.9%), Ent. casseliflavus (5.5%), Ent. faecium (5.2%) and Ent. sulfureus (0.1%). The vast majority of the strains (69.7%) formed a homogeneous 16S rDNA genotype that differed from those of known enterococci. A screening for antagonistic activity using an agar spot test revealed that 18.4% of all isolates were potential antagonists. Partially-purified proteins extracted from cell-free culture supernatant fluids of various species were characterized as pH- and heat-stable bacteriocins active against a wide range of lactic acid bacteria, clostridia and Lister ia. The producing strains were antagonistically active even on 'phylloplane agar' at temperatures between 4 and 37 degreesC. Conclusions: Enterococci are a common part of the epiphytic microflora of grasses, displaying probably some antagonistic activity. Significance and Impact of the Study: The results provide new information on the distribution, species diversity and antagonistic potential of enterococci in the phyllosphere.
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页码:54 / 66
页数:13
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