The Diversity of Polyketide Synthase Genes from Sugarcane-Derived Fungi

被引:10
作者
Rojas, Juan Diego [1 ]
Sette, Lara Duraes [2 ]
de Araujo, Welington L. [1 ]
Garcez Lopes, Mateus Schreiner [1 ]
da Silva, Luiziana Ferreira [1 ]
Furlan, Renata L. A. [1 ]
Padilla, Gabriel [1 ]
机构
[1] Univ Sao Paulo, Inst Ciencias Biomed ICB, BR-05508900 Sao Paulo, Brazil
[2] Univ Estadual Campinas UNICAMP, Div Recursos Microbianos CPQBA, BR-13081970 Campinas, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
FILAMENTOUS FUNGI; ENDOPHYTIC BACTERIA; CRYSTAL-STRUCTURE; FATTY-ACIDS; DOMAINS; IDENTIFICATION; BIOSYNTHESIS; METHYLTRANSFERASES; INHIBITION; EXPRESSION;
D O I
10.1007/s00248-011-9938-0
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The chemical ecology and biotechnological potential of metabolites from endophytic and rhizosphere fungi are receiving much attention. A collection of 17 sugarcane-derived fungi were identified and assessed by PCR for the presence of polyketide synthase (PKS) genes. The fungi were all various genera of ascomycetes, the genomes of which encoded 36 putative PKS sequences, 26 shared sequence homology with beta-ketoacyl synthase domains, while 10 sequences showed homology to known fungal C-methyltransferase domains. A neighbour-joining phylogenetic analysis of the translated sequences could group the domains into previously established chemistry-based clades that represented non-reducing, partially reducing and highly reducing fungal PKSs. We observed that, in many cases, the membership of each clade also reflected the taxonomy of the fungal isolates. The functional assignment of the domains was further confirmed by in silico secondary and tertiary protein structure predictions. This genome mining study reveals, for the first time, the genetic potential of specific taxonomic groups of sugarcane-derived fungi to produce specific types of polyketides. Future work will focus on isolating these compounds with a view to understanding their chemical ecology and likely biotechnological potential.
引用
收藏
页码:565 / 577
页数:13
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