Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A

被引：33

作者：

Atoui, Ali ^{[1
]}

Dao, Huy Phong ^{[1
]}

Mathieu, Florence ^{[1
]}

Lebrihi, Ahmed ^{[1
]}

机构：

[1] Inst Natl Polytech Toulouse, Ecole Natl Super Agron Toulouse, Dept Bioprocedes & Syst Microbiens, CNRS,UMR5503,Lab Genie Chim,UPS,INPT, F-31326 Castanet Tolosan, France

来源：

MOLECULAR NUTRITION & FOOD RESEARCH | 2006年 / 50卷 / 06期

关键词：

Aspergillus carbonarius; Aspergillus ochraceus; mycotoxins; ochratoxin A; polyketide synthase;

D O I：

10.1002/mnfr.200500165

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The diversity of polyketide synthase (PKS) genes in Aspergillus ochraceus NRRL 3174 and Aspergillus carbonarius 2Mu134 has been investigated using different primer pairs previously developed for the ketosynthase (KS) domain of fungal PKSs. Nine different KS domain sequences in A. ochraceus NRRL 3174 as well as five different KS domain sequences in A. carbonarius 2Mu134 have been identified. The identified KS fragments were distributed in five different clusters on the phylogenetic tree, indicating that they most probably represent PKSs responsible for different functions.

引用

页码：488 / 493

页数：6

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