Effect of 13C-, 18O- and 2H-labeling on the infrared modes of UV-induced phenoxyl radicals

被引：19

作者：

Berthomieu, C ^{[1
]}

Boullais, C

Neumann, JM

Boussac, A

机构：

[1] CEA Saclay, Dept Biol Cellulaire & Mol, Sect Bioenerget, F-91191 Gif Sur Yvette, France

[2] CEA Saclay, Dept Biol Cellulaire & Mol, Serv Mol Marquees, F-90091 Gif Sur Yvette, France

[3] CEA Saclay, Dept Biol Cellulaire & Mol, Sect Biophys Prot & Membranes, F-91191 Gif Sur Yvette, France

[4] CEA Saclay, Dept Biol Cellulaire & Mol, URA CNRS 2096, F-91191 Gif Sur Yvette, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1998年 / 1365卷 / 1-2期

关键词：

infrared spectrum; tyrosine; radical; isotope-labeling;

D O I：

10.1016/S0005-2728(98)00056-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure and environment of redox active tyrosines present in several metalloenzymes can be studied by resonance Raman spectroscopy or Fourier transform infrared difference spectroscopy. Assignments of the vibrational modes in vivo often requires in vitro studies on model compounds. This approach is briefly reviewed. New results are shown on the influence of isotope-labeling on the infrared spectra of tyrosine, p-methylphenol and phenol radicals obtained in vitro by W-irradiation. The infrared spectra of the radicals are dominated by the v(CO) mode at 1515-1504 cm(-1). The frequency shifts induced on this mode by C-13- H-2-, and O-18-labeling are reported. (C) 1998 Elsevier Science B.V.

引用

页码：112 / 116

页数：5

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