Field evidence for mechanical transmission of rabbit haemorrhagic disease virus (RHDV) by flies (Diptera: Calliphoridae) among wild rabbits in Australia

被引：93

作者：

Asgari, S

Hardy, JRE

Sinclair, RG

Cooke, BD

机构：

[1] Dept Crop Protect, Glen Osmond, SA 5064, Australia

[2] Anim & Plant Control Commiss, Adelaide, SA 5001, Australia

[3] CSIRO, Div Wildlife & Ecol, Canberra, ACT 2601, Australia

来源：

VIRUS RESEARCH | 1998年 / 54卷 / 02期

关键词：

RHDV; rabbit haemorrhagic disease; RT-PCR; flyspots; transmission;

D O I：

10.1016/S0168-1702(98)00017-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Field collected flies were screened for the presence of rabbit haemorrhagic disease virus (RHDV) by applying reverse transcriptase PCR (RT-PCR) in which primers specific to the capsid protein of the virus were used. The virus was detected in flies from locations where rabbit haemorrhagic disease (RHD) was reported and also soon after the release of RHDV in a 'clean' area. Oral and/or anal excretions of flies (flyspots) were found to contain viable virus and oral inoculation of rabbits revealed that a single flyspot was able to cause RHD. We conclude that flyspots are a major potential source of the virus for oral or conjunctival transmission of the virus to rabbits. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 132

页数：10

共 26 条

[11] The complete nucleotide sequence of rabbit haemorrhagic disease virus (Czech strain V351): Use of the polymerase chain reaction to detect replication in Australian vertebrates and analysis of viral population sequence variation [J].