Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium

被引：44

作者：

Risitano, A ^{[1
]}

Fox, KR ^{[1
]}

机构：

[1] Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants, England

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 08期

关键词：

G-quartet; quadruplex; fluorescence; molecular beacon; DNA melting; inosine;

D O I：

10.1016/j.bmcl.2005.02.050

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We have examined the stability of fluorescently-labelled oligonucleotides that are based on the human telomeric repeat [(GGGTTA)(3)GGG], in which one of the guanines in turn is substituted with inosine. We show that the relative stability of the substitutions is different in the presence of sodium and potassium. The data for potassium suggest a parallel arrangement of the strands, while the sodium form is mixed parallel and antiparallel. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2047 / 2050

页数：4

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