Therapeutic antibody expression technology

被引:134
作者
Chadd, HE [1 ]
Chamow, SM [1 ]
机构
[1] Abgenix Inc, Dept Proc Sci, Fremont, CA 94555 USA
关键词
D O I
10.1016/S0958-1669(00)00198-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
With the technological advances made during the past decade, antibodies now represent an important and growing class of biotherapeutics. With the potential new targets resulting from genomics and with methods now in place to make fully human antibodies, the potential of antibodies as valuable therapeutics in oncology, inflammation and cardiovascular disease can be fully realised. Systems to produce these antibodies as full-length molecules and as fragments include expression in both mammalian and bacterial cells grown in bioreactors and in transgenic organisms. Factors including molecular fidelity and the cost of goods are critical in evaluating expression systems. Mammalian cell culture and transgenic organisms show the greatest promise for the expression of full-length, recombinant human antibodies, and bacterial fermentation seems most favorable for the expression of antibody fragments.
引用
收藏
页码:188 / 194
页数:7
相关论文
共 48 条
  • [1] Baez J, 2000, BIOPHARM-APPL T BIO, V13, P50
  • [2] Baselga J, 1998, CANCER RES, V58, P2825
  • [3] HIGH-LEVEL EXPRESSION OF A RECOMBINANT ANTIBODY FROM MYELOMA CELLS USING A GLUTAMINE-SYNTHETASE GENE AS AN AMPLIFIABLE SELECTABLE MARKER
    BEBBINGTON, CR
    RENNER, G
    THOMSON, S
    KING, D
    ABRAMS, D
    YARRANTON, GT
    [J]. BIO-TECHNOLOGY, 1992, 10 (02): : 169 - 175
  • [4] BLAZAR B, 2000, BIOL BLOOD MARROW TR, V6, P133
  • [5] THE STRUCTURAL REQUIREMENTS FOR COMPLEMENT ACTIVATION BY IGG - DOES IT HINGE ON THE HINGE
    BREKKE, OH
    MICHAELSEN, TE
    SANDLIE, I
    [J]. IMMUNOLOGY TODAY, 1995, 16 (02): : 85 - 90
  • [6] Chamow S, 1999, ANTIBODY FUSION PROT
  • [7] Therapeutic antibody fragments with prolonged in vivo half-lives
    Chapman, AP
    Antoniw, P
    Spitali, M
    West, S
    Stephens, S
    King, DJ
    [J]. NATURE BIOTECHNOLOGY, 1999, 17 (08) : 780 - 783
  • [8] Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris
    Cupit, PM
    Whyte, JA
    Porter, AJ
    Browne, MJ
    Holmes, SD
    Harris, WJ
    Cunningham, C
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1999, 29 (05) : 273 - 277
  • [9] Transgenic mice as a source of fully human antibodies for the treatment of cancer
    Davis, CG
    Gallo, ML
    Corvalan, JRF
    [J]. CANCER AND METASTASIS REVIEWS, 1999, 18 (04) : 421 - 425
  • [10] Chimeric anti-CD20 (IDEC-C2B8) monoclonal antibody sensitizes a B cell lymphoma cell line to cell killing by cytotoxic drugs
    Demidem, A
    Lam, T
    Alas, S
    Hariharan, K
    Hanna, N
    Bonavida, B
    [J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 1997, 12 (03) : 177 - 186