Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris

被引:9
作者
Cupit, PM
Whyte, JA
Porter, AJ
Browne, MJ
Holmes, SD
Harris, WJ
Cunningham, C
机构
[1] Univ Aberdeen, Inst Med Sci, Dept Mol & Cell Biol, Aberdeen AB9 1FX, Scotland
[2] SmithKline Beecham Pharmaceut, Res & Dev, Harlow, Essex, England
关键词
D O I
10.1046/j.1472-765X.1999.00600.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The potential of recombinant antibody fragments is likely to be fulfilled only if they can be produced routinely at high concentrations. We have compared the ability of Escherichia coli and Pichia pastoris to produce functional recombinant single chain antibody (scAb) fragments. Two scAb fragments were expressed, an antihuman type V acid phosphatase (TRAP) and an anti-Pseudomonas aeruginosa lipoprotein I. We report here that, while expression from P. pastoris resulted in a significantly increased level of expression of the anti-TRAP scAb compared to E. coli, neither fragment was able to bind its target antigen as well as the bacterial product.
引用
收藏
页码:273 / 277
页数:5
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