High level secretion of single-chain antibody in Pichia expression system

被引：11

作者：

Luo, D

Mah, N

Krantz, M

Wishart, D

Jacobs, F

Martin, L

机构：

[1] BIOMIRA INC,EDMONTON,AB T6N 1H1,CANADA

[2] UNIV ALBERTA,DEPT BIOCHEM,EDMONTON,AB T6G 2H7,CANADA

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 10期

关键词：

D O I：

10.1023/A:1018404720349

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single chain Fv-170 (scFv-170) was constructed and expressed in the methylotrophic yeast Pichia pastoris. Recombinant scFV-170 was highly expressed in an alpha F-signal based expression Vector and sufficiently secreted from the cells in protein-free medium. Further, the scFv-170 maintained the intrinsic binding activity and exhibited the same binding pattern as that of MAb 170H.82, as measured in competitive radioimmunoassay (RIA) experiments.

引用

页码：759 / 761

页数：3

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