Rapid and efficient microwave-assisted sulfate modification of lentinan and its antioxidant and antiproliferative activities in vitro

被引：80

作者：

Feng, Yili ^{[1
]}

Li, Weiqi ^{[1
]}

Wu, Xueqian ^{[2
]}

He, Liang ^{[2
]}

Ma, Suyun ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, Inst Microbiol, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Forestry Acad, Key Lab Biol & Chem Utilizat Forest Resources, Hangzhou 310023, Zhejiang, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 82卷 / 03期

基金：

浙江省自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Lentinan; Sulfation; Microwave; Structure; Antioxidant and antiproliferative activities; LOW-MOLECULAR-WEIGHT; FREE-RADICALS; LIPID-PEROXIDATION; POLYSACCHARIDE FRACTION; ANTITUMOR ACTIVITIES; GANODERMA-LUCIDUM; HL-60; CELLS; EDODES; LEUKEMIA; GROWTH;

D O I：

10.1016/j.carbpol.2010.05.025

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Lentinan (LNT) is a highly functional biomacromolecule, and its sulfated derivatives (sLNT) exhibit improved pharmacological properties compared to LNT. In the present study. LNT extracted from the fermentation broth of Lentinus edodes was sulfated using two methods, conventional heating and novel microwave radiation, and their biological activities were evaluated using an antioxidant system and an antiproliferation cell model. Our results indicate that the two sulfated derivates have similar physicochemical properties and spectroscopic profiles. Nevertheless, microwave radiation produced a higher yield of sLNT, with a shorter processing time and lower degradation. In contrast to LNT, the sLNT derivatives exhibited a strong antioxidant activity in vitro. In addition, the derivatives exhibited a significant antiproliferative effect on leukemia cells, whereas LNT exhibited the opposite outcome. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：605 / 612

页数：8

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