Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS

被引：37

作者：

Clarke, NJ

Tomlinson, AJ

Ohyagi, Y

Younkin, S

Naylor, S

机构：

[1] Mayo Clin Jacksonville, Dept Pharmacol, Jacksonville, FL 32224 USA

[2] Mayo Clin & Mayo Fdn, Dept Biochem & Mol Biol, Biomed Mass Spect Facil, Rochester, MN 55905 USA

[3] Iizuka Hosp, Dept Neurol, Iizuka, Fukuoka 820, Japan

[4] Mayo Clin & Mayo Fdn, Dept Pharmacol, Rochester, MN 55905 USA

[5] Mayo Clin & Mayo Fdn, Clin Pharmacol Unit, Rochester, MN 55905 USA

来源：

FEBS LETTERS | 1998年 / 430卷 / 03期

关键词：

Alzheimer's disease; amyloid beta; HPLC; immunoprecipitation; mass spectrometry;

D O I：

10.1016/S0014-5793(98)00706-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitative method for detection of amyloid beta peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described, Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of A beta 1-40 and A beta 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for A beta 1-40 and A beta 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other A beta peptide species that may also play a role in the onset of AD in humans. (C) 1998 Federation of European Biochemical Societies.

引用

页码：419 / 423

页数：5

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