Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays

被引：36

作者：

Levi, Jelena ^{[1
]}

De, Abhijit ^{[1
]}

Cheng, Zhen ^{[1
]}

Gambhir, Sanjiv Sam ^{[1
]}

机构：

[1] Stanford Univ, MIPS, Dept Radiol & Bioengn, Biox Program, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 39期

关键词：

D O I：

10.1021/ja073936h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report here syntheses of bisdeoxycoelenterazine derivatives that show significantly improved kinetics of the bioluminescent reaction. All derivatives contain a protecting group at the carbonyl group of the imidazopyrazinone moiety that ensures slower light signal decay. The longer lasting light signal offers the possibility of real-time imaging of protein-protein interactions.

引用

页码：11900 / +

页数：3

共 16 条

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