Expression in Escherichia coli of a new multidrug efflux pump, MexXY, from Pseudomonas aeruginosa

被引：214

作者：

Mine, T

Morita, Y

Kataoka, A

Mizushima, T

Tsuchiya, T ^{[1
]}

机构：

[1] Okayama Univ, Fac Pharmaceut Sci, Dept Microbiol, Okayama 7008530, Japan

[2] Okayama Univ, Ctr Gene Res, Okayama 7008530, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 02期

关键词：

D O I：

10.1128/AAC.43.2.415

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two new genes (mexXY) similar to mexAB, mexCD, and mexEF and mediating multidrug resistance were cloned from the chromosome of Pseudomonas aeruginosa. Elevated ethidium extrusion was observed with Escherichia coli cells harboring the plasmid carrying mexXY. This MexXY system confers higher resistance to fluoroquinolones than the MexAB and MexCD systems, and E. coli TolC or P. aeruginosa OprM is necessary for the function of the MexXY system.

引用

页码：415 / 417

页数：3

共 26 条

[11] GENES ACRA AND ACRB ENCODE A STRESS-INDUCED EFFLUX SYSTEM OF ESCHERICHIA-COLI
MA, D
COOK, DN
ALBERTI, M
PON, NG
NIKAIDO, H
HEARST, JE
[J]. MOLECULAR MICROBIOLOGY, 1995, 16 (01) : 45 - 55
[12] Ma Dzwokai, 1994, Trends in Microbiology, V2, P489, DOI 10.1016/0966-842X(94)90654-8
[13] NorM, a putative multidrug efflux protein, of Vibrio parahaemolyticus and its homolog in Escherichia coli
Morita, Y
Kodama, K
Shiota, S
Mine, T
Kataoka, A
Mizushima, T
Tsuchiya, T
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (07) : 1778 - 1782
[14] DNA-SEQUENCE OF THE GENE FOR THE OUTER-MEMBRANE LIPOPROTEIN OF ESCHERICHIA-COLI - EXTREMELY AT-RICH PROMOTER
NAKAMURA, K
INOUYE, M
[J]. CELL, 1979, 18 (04) : 1109 - 1117
[15] Multidrug efflux pumps of gram-negative bacteria
Nikaido, H
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (20) : 5853 - 5859
[16] MULTIPLE ANTIBIOTIC-RESISTANCE IN PSEUDOMONAS-AERUGINOSA - EVIDENCE FOR INVOLVEMENT OF AN EFFLUX OPERON
POOLE, K
KREBES, K
MCNALLY, C
NESHAT, S
[J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (22) : 7363 - 7372
[17] CLONING AND SEQUENCE-ANALYSIS OF AN ENVCD HOMOLOG IN PSEUDOMONAS-AERUGINOSA - REGULATION BY IRON AND POSSIBLE INVOLVEMENT IN THE SECRETION OF THE SIDEROPHORE PYOVERDINE
POOLE, K
HEINRICHS, DE
NESHAT, S
[J]. MOLECULAR MICROBIOLOGY, 1993, 10 (03) : 529 - 544
[18] Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa
Poole, K
Gotoh, N
Tsujimoto, H
Zhao, QX
Wada, A
Yamasaki, T
Neshat, S
Yamagishi, JI
Li, XZ
Nishino, T
[J]. MOLECULAR MICROBIOLOGY, 1996, 21 (04) : 713 - 724
[19] Sambrook J., 2002, MOL CLONING LAB MANU
[20] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS
SANGER, F
NICKLEN, S
COULSON, AR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) : 5463 - 5467

← 1 2 3 →