eSGA:: E. coli synthetic genetic array analysis

被引：191

作者：

Butland, Gareth ^{[1
,2
]}

Babu, Mohan ^{[1
]}

Diaz-Mejia, J. Javier ^{[1
,3
]}

Bohdana, Fedyshyn ^{[1
]}

Phanse, Sadhna ^{[1
]}

Gold, Barbara ^{[2
]}

Yang, Wenhong ^{[2
]}

Li, Joyce ^{[1
]}

Gagarinova, Alla G. ^{[4
]}

Pogoutse, Oxana ^{[1
]}

Mori, Hirotada ^{[5
]}

Wanner, Barry L. ^{[5
]}

Lo, Henry ^{[1
]}

Wasniewski, Jas ^{[1
]}

Christopoulos, Constantine ^{[1
]}

Ali, Mehrab ^{[4
]}

Venn, Pascal ^{[1
]}

Safavi-Naini, Anahita ^{[1
]}

Sourour, Natalie ^{[1
]}

Caron, Simone ^{[1
]}

Choi, Ja-Yeon ^{[1
]}

Laigle, Ludovic ^{[1
]}

Nazarians-Armavil, Anaies ^{[1
]}

Deshpande, Avnish ^{[1
]}

Joe, Sarah ^{[1
]}

Datsenko, Kirill A. ^{[6
]}

Yamamoto, Natsuko ^{[5
]}

Andrews, Brenda J. ^{[1
,4
]}

Boone, Charles ^{[1
,5
]}

Ding, Huiming ^{[1
]}

Sheikh, Bilal ^{[1
]}

Moreno-Hagelsieb, Gabriel ^{[3
]}

Greenblatt, Jack F. ^{[1
,4
]}

Emili, Andrew ^{[1
,4
]}

机构：

[1] Univ Toronto, Banting & Best Dept Med Res, Terrence Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada

[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA

[3] Wilfrid Laurier Univ, Dept Biol, Waterloo, ON N2L 3C5, Canada

[4] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

[5] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan

[6] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/NMETH.1239

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Physical and functional interactions define the molecular organization of the cell. Genetic interactions, or epistasis, tend to occur between gene products involved in parallel pathways or interlinked biological processes. High-throughput experimental systems to examine genetic interactions on a genome-wide scale have been devised for Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans and Drosophila melanogaster, but have not been reported previously for prokaryotes. Here we describe the development of a quantitative screening procedure for monitoring bacterial genetic interactions based on conjugation of Escherichia coli deletion or hypomorphic strains to create double mutants on a genome-wide scale. The patterns of synthetic sickness and synthetic lethality (aggravating genetic interactions) we observed for certain double mutant combinations provided information about functional relationships and redundancy between pathways and enabled us to group bacterial gene products into functional modules.

引用

页码：789 / 795

页数：7

共 30 条

[1] Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants:: the Keio collection [J].