Characterization of polysaccharide from Astragalus radix as the macrophage stimulator

被引：192

作者：

Zhao, Lu-Hang ^{[1
]}

Ma, Zhi-Xin ^{[1
]}

Zhu, Jie ^{[2
]}

Yu, Xiao-Hong ^{[3
]}

Weng, Deng-Po ^{[3
]}

机构：

[1] Zhejiang Univ, Dept Biochem & Genet, Sch Med, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Hosp, Dept Clin Lab, Hangzhou 310013, Zhejiang, Peoples R China

[3] Zhejiang Univ, Expt Ctr Mol Cell Biol, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CELLULAR IMMUNOLOGY | 2011年 / 271卷 / 02期

关键词：

APS; Macrophage; NO; TNF-alpha; GM-CSF; ANTITUMOR-ACTIVITY; NITRIC-OXIDE; IN-VITRO; MEMBRANACEUS; CELLS; IMMUNOMODULATION; ROOTS;

D O I：

10.1016/j.cellimm.2011.07.011

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Astragalus polysaccharide (APS) was obtained by hot water extraction, alcohol precipitation, gel-permeation chromatography and ultrafiltration. Fluorescence material 2-aminoacridone (2-AMAC) labeled APS bind to macrophage in a time- dependent manner and the binding can be remarkably inhibited by APS. Furthermore, the effect of APS on RAW264.7 macrophage demonstrated APS increase the level of cytokines including TNF-alpha, GM-CSF and the production of NO. NF-kappa B protein levels are increased in response to APS. Blocking NF-kappa B with specific inhibitor resulted in decreased levels of NO and TNF-alpha. The results suggested that APS possess potent immunomodulatory activity by stimulating macrophage and could be used as an immunotherapeutic adjuvant. (C) 2011 Published by Elsevier Inc.

引用

页码：329 / 334

页数：6

共 32 条

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