Different contributions of side-chains in β-D-(1 → 3,6)-galactans on intestinal Peyer's patch-immunomodulation by polysaccharides from Astragalus mongholics Bunge

被引：51

作者：

Kiyohara, Hiroaki ^{[1
,2
]}

Uchida, Taichi ^{[1
]}

Takakiwa, Manami ^{[2
]}

Matsuzaki, Toshiake ^{[1
]}

Hada, Noriyasu ^{[3
]}

Takeda, Tadahiro ^{[3
]}

Shibata, Toshiro ^{[4
]}

Yamada, Haruki ^{[1
,2
]}

机构：

[1] Kitasato Univ, Kitasato Inst Life Sci, Dept Drug Discovery Sci, Minato Ku, Tokyo 1088641, Japan

[2] Kitasato Univ, Oriental Med Res Ctr, Minato Ku, Tokyo 1088641, Japan

[3] Keio Univ, Fac Pharm, Div Nat Med, Minato Ku, Tokyo 1058512, Japan

[4] Natl Inst Biochem Innovat, Hokkaido Div, Res Ctr Med Plant Resources, Nayoro, Hokkaido 0960065, Japan

来源：

PHYTOCHEMISTRY | 2010年 / 71卷 / 2-3期

关键词：

Astragalus mongholics Bunge; Leguminosae; Polysaccharides, beta-D-(1 -> 3,6)-Galactan; Oligosaccharide side-chain; Enzymatic digestion; Exo-beta-D-(1 -> 3)-galactanase; High performance anion-exchange chromatography Immunomodulating activity; Intestinal immune system; Peyer's patches; CELL-WALL POLYSACCHARIDE; RHAMNOGALACTURONAN-II; PECTIC POLYSACCHARIDES; ANTIGENIC EPITOPE; PURIFICATION; SYSTEM; RHIZOMES;

D O I：

10.1016/j.phytochem.2009.10.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thirteen polysaccharides isolated from an extract of the aerial portions of Astragalus mongholics Bunge demonstrated immunomodulating activity against Peyer's patch immunocompetent cells. Nine of the active polysaccharide fractions were composed of either arabinogalactans, pectic arabinogalactans or pectins. The activities of the arabinogalactans and pectic arabinogalactans were associated with beta-D(1 -> 3)-galactan moieties branched with beta-D-(1 -> 6)-galactooligosaccharide side-chains having degrees of polymerization of 8 or less. Degradation of the beta-D-(1 -> 3)-galactan or beta-D-(1 -> 6)-galactosyl side-chains in the arabinogalactans significantly decreased immunomodulating activity. Rhamnogalacturonan I (RG-I) with beta-D-(1 -> 3,6)-galactosyl side-chains having terminal beta-D-GlcA showed activity in the pectin-enriched fractions. Interestingly, the terminal GlcA was not required for activity of the arabinogalactan-enriched fractions, suggesting at least two different immunomodulating structures. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：280 / 293

页数：14

共 31 条

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