Polymorphisms in Trypanosoma cruzi: Evidence of genetic recombination

被引：78

作者：

Bogliolo, AR

LauriaPires, L

Gibson, WC

机构：

[1] UNIV BRASILIA, FAC CIENCIAS SAUDE, LAB MULTIDISCIPLINAR D DE CHAGAS, BR-70910900 BRASILIA, DF, BRAZIL

[2] UNIV BRISTOL, SCH VET, DEPT PATHOL, LANGFORD, ENGLAND

来源：

ACTA TROPICA | 1996年 / 61卷 / 01期

基金：

英国医学研究理事会;

关键词：

Trypanosoma cruzi; isoenzyme; restriction fragment length polymorphism; ploidy; genetic exchange;

D O I：

10.1016/0001-706X(95)00138-5

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The ploidy of Trypanosoma cruzi is until now undetermined although analysis of isoenzymes, molecular karyotype and DNA content suggest diploidy in a very plastic genome. Also, there has been no convincing demonstration of genetic exchange and it has been proposed that reproduction is clonal. We have compared 18 T. cruzi stocks and clones from the same area or host by means of isoenzyme analysis (12 loci) and restriction site polymorphisms in and around three glycolytic genes (glyceraldehyde-3-phosphate dehydrogenase, aldolase and glucosephosphate isomerase). The analysis demonstrated the presence of homozygotes and heterozygotes and is compatible with diploidy for these housekeeeping genes. This strongly supports the hypothesis of genetic exchange in T. cruzi and further elucidates the genetic diversity within natural T. cruzi populations.

引用

页码：31 / 40

页数：10

共 41 条

[1] MOLECULAR-CLONING AND ANALYSIS OF 2 TANDEMLY LINKED GENES FOR PYRUVATE-KINASE OF TRYPANOSOMA-BRUCEI [J].