Low level Pb2+ exposure affects hippocampal protein kinase Cγ gene and protein expression in rats

被引：36

作者：

Nihei, MK ^{[1
]}

McGlothan, JL ^{[1
]}

Toscano, CD ^{[1
]}

Guilarte, TR ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA

来源：

NEUROSCIENCE LETTERS | 2001年 / 298卷 / 03期

关键词：

protein kinase C gamma; hippocampus; Pb2+; back-phosphorylation; protein kinase C activity; H-3]phorbol 12,13 dibutyrate binding;

D O I：

10.1016/S0304-3940(00)01741-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We examined the effect of chronic exposure to lead (Pb2+) on protein kinase C (PKC) in 50-day-old rat hippocampus. Cytosolic and membrane fractions of hippocampus from Pb2+-exposed rats showed reduced expression of PKC gamma protein. In contrast, a significant elevation of PKC gamma mRNA was observed in pyramidal and dentate granule cell layers. Protein expression of alpha, betaI, beta II and epsilon isoenzymes were unchanged in Pb2+-exposed rats, as was [H-3]phorbol 12,13 dibutyrate (PDBu) binding in tissue slices. Differences were not observed in Ca2+-dependent or -independent PKC activity, or in PKC-specific back-phosphorylation of hippocampal homogenates from Pb2+-exposed rats. Reduced subcellular levels of PKC gamma in Pb2+-exposed rats suggest that signal transduction in the hippocampus may be selectively altered and may be important in manifesting Pb2+-induced impairments of synaptic plasticity, learning and memory. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：212 / 216

页数：5

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