New method to enhance ajmalicine production in Catharanthus roseus cell cultures based on the use of cyclodextrins

被引：38

作者：

Almagro, L. ^{[1
]}

Lopez Perez, A. J. ^{[1
]}

Pedreno, M. A. ^{[1
]}

机构：

[1] Univ Murcia, Dept Plant Biol, Fac Biol, E-30100 Murcia, Spain

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 02期

关键词：

Ajmalicine; Catharanthus roseus; Cell culture; Cyclodextrin; Elicitation; Methyl jasmonate; ALKALOID PRODUCTION;

D O I：

10.1007/s10529-010-0430-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The joint use of cyclodextrins and methyljasmonate, when accompanied by a short exposure to UV, enhanced extracellular ajmalicine accumulation to 1040 +/- A 26.6 mg/l in suspension cultured cells of Catharanthus roseus. The success of this strategy is due to the use of cyclodextrins, which not only induce ajmalicine biosynthesis but also promote adduct formation. This removes ajmalicine from the medium, reduces feedback inhibition and ajmalicine degradation, and allows its accumulation in the culture medium at elevated concentrations.

引用

页码：381 / 385

页数：5

共 12 条

[1] Modified cyclodextrins are chemically defined glucan inducers of defense responses in grapevine cell cultures [J].

Bru, R ;

Sellés, S ;

Casado-Vela, J ;

Belchí-Navarro, S ;

Pedreño, MA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (01) :65-71

[2]

Lee CWT, 2000, BIOTECHNOL BIOENG, V67, P61, DOI 10.1002/(SICI)1097-0290(20000105)67:1<61::AID-BIT7>3.0.CO

[3]

2-J

[4] Enhancement of ajmalicine production in Catharanthus roseus cell cultures with methyl jasmonate is dependent on timing and dosage of elicitation [J].

Lee-Parsons, CWT ;

Ertürk, S ;

Tengtrakool, J .

BIOTECHNOLOGY LETTERS, 2004, 26 (20) :1595-1599

[5] ORGANIC GROWTH FACTOR REQUIREMENTS OF TOBACCO TISSUE CULTURES [J].

LINSMAIER, EM ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1965, 18 (01) :100-+

[6] INDOLE ALKALOID FORMATION AND STORAGE IN CELL-SUSPENSION CULTURES OF CATHARANTHUS-ROSEUS [J].

NEUMANN, D ;

KRAUSS, G ;

HIEKE, M ;

GROGER, D .

PLANTA MEDICA, 1983, 48 (01) :20-23

[7]

Ramani Shilpa, 2008, J Mol Signal, V3, P9, DOI 10.1186/1750-2187-3-9

[8]

van der Heijden R, 2004, CURR MED CHEM, V11, P607

[9] Synergistic action between jasmonic acid and nitric oxide in inducing matrine accumulation of Sophora flavescens suspension cells [J].

Xu, Mao-Jun ;

Dong, Ju-Fang .

JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2008, 50 (01) :92-101

[10] Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor [J].

Zamboni, Anita ;

Gatto, Pamela ;

Cestaro, Alessandro ;

Pilati, Stefania ;

Viola, Roberto ;

Mattivi, Fulvio ;

Moser, Claudio ;

Velasco, Riccardo .

BMC GENOMICS, 2009, 10 :363

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