Enhancement of ajmalicine production in Catharanthus roseus cell cultures with methyl jasmonate is dependent on timing and dosage of elicitation

被引：54

作者：

Lee-Parsons, CWT

Ertürk, S

Tengtrakool, J

机构：

[1] Northeastern Univ, Dept Chem Engn, Snell Engn Ctr 342, Boston, MA 02115 USA

[2] Genzyme Corp, Bioengn, Framingham, MA 01701 USA

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 20期

基金：

美国国家科学基金会;

关键词：

ajmalicine; alkaloid; Catharanthus roseus; elicitation; methyl jasmonate;

D O I：

10.1023/B:BILE.0000045825.37395.94

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The optimum growth stage for enhancing ajmalicine production in Catharanthus roseus cultures with methyl jasmonate (MJ) was after 6 d growth. MJ added at 10 or 100 mum on day 6 gave a maximum ajmalicine production of 10.2 mg 1(-1), a 300% increase over that of non-elicited cultures.

引用

页码：1595 / 1599

页数：5

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