AmpC β-lactamases in Escherichia coli: emergence of CMY-2-producing virulent phylogroup D isolates belonging mainly to STs 57, 115, 354, 393, and 420, and phylogroup B2 isolates belonging to the international clone O25b-ST131

被引:40
作者
Oteo, Jesus [1 ]
Cercenado, Emilia [2 ]
Cuevas, Oscar [1 ]
Bautista, Veronica [1 ]
Delgado-Iribarren, Alberto [3 ]
Orden, Beatriz [4 ]
Perez-Vazquez, Maria [1 ]
Garcia-Cobos, Silvia [1 ]
Campos, Jose [1 ,5 ]
机构
[1] Inst Salud Carlos III, Ctr Nacl Microbiol, Bacteriol Serv, Antibiot Lab, Madrid 28220, Spain
[2] Hosp Gen Gregorio Maranon, Dept Microbiol, Madrid 28007, Spain
[3] Hosp Fdn Alcorcon, Dept Microbiol, Madrid 28922, Spain
[4] Hosp Puerta Hierro, Ctr Especialidades Arguelles, Dept Microbiol, Madrid 28760, Spain
[5] CSIC, Madrid 28006, Spain
关键词
AmpC; Escherichia coli; CMY-2; MOLECULAR CHARACTERIZATION; KLEBSIELLA-PNEUMONIAE; RESISTANCE; PREVALENCE; STRAINS; SURVEILLANCE; COMMUNITY; SPREAD;
D O I
10.1016/j.diagmicrobio.2010.02.008
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Cephalosporins resistance is increasing in Escherichia coli in Spain. We characterize infections by E. coli with reduced susceptibility to third-generation cephalosporins (3GCs) with the AmpC phenotype. Between January 2004 and March 2007, 121 E. coli isolates with the AmpC phenotype were collected (4.8% of all the 2538 E. coli isolates with reduced susceptibility to 3GCs). These isolates were further characterized by clinical and molecular analysis. Plasmid-encoded ampC genes were detected in 46 (38%) isolates (43 CMY-2); 75 isolates (62%) had modifications in the chromosomal ampC promoter region (c-AmpC). CMY-2 producers belonged primarily to the more virulent phylogroup D (48.4%), whereas most isolates of c-AmpC belonged to phylogroup A (56.4%). Bacteremia and infections in children were more frequently produced by CMY-2 producers. CMY-2-producing phylogroup D E. coli belonged to 8 multilocus sequence typing types. Three CMY-2 producers belonged to O25b/ST131/B2 clone. Infections caused by E. coli with the AmpC phenotype may be spreading primarily because of CMY-2-producing phylogroup D isolates, although this enzyme was also detected in the O25b/ST131/B2 clone. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:270 / 276
页数:7
相关论文
共 29 条
[1]  
[Anonymous], 2010, European Antimicrobial Resistance Surveillance System
[2]   Characterization of plasmids encoding CMY-2 AmpC β-lactamases from Escherichia coli in Canadian intensive care units [J].
Baudry, Patricia J. ;
Mataseje, Laura ;
Zhanel, George G. ;
Hoban, Daryl J. ;
Mulvey, Michael R. .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2009, 65 (04) :379-383
[3]   Analysis of the effects of-42 and-32 ampC promoter mutations in clinical isolates of Escherichia coli hyperproducing AmpC [J].
Caroff, N ;
Espaze, E ;
Gautreau, D ;
Richet, H ;
Reynaud, A .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 45 (06) :783-788
[4]   Rapid and simple determination of the Escherichia coli phylogenetic group [J].
Clermont, O ;
Bonacorsi, S ;
Bingen, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4555-4558
[5]   The CTX-M-15-producing Escherichia coli diffusing clone belongs to a highly virulent B2 phylogenetic subgroup [J].
Clermont, Olivier ;
Lavollay, Marie ;
Vimont, Sophie ;
Deschamps, Catherine ;
Forestier, Christiane ;
Branger, Catherine ;
Denamur, Erick ;
Arlet, Guillaume .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2008, 61 (05) :1024-1028
[6]  
Clinical and Laboratory Standards Institute (CLSI), 2007, M100S17 CLSI, V27
[7]   Dissemination of clonally related Escherichia coli strains expressing extended-spectrum β-lactamase CTX-M-15 [J].
Coque, Teresa M. ;
Novais, Angela ;
Carattoli, Alessandra ;
Poirel, Laurent ;
Pitout, Johann ;
Peixe, Luisa ;
Baquero, Fernando ;
Canton, Rafael ;
Nordmannj, Patrice .
EMERGING INFECTIOUS DISEASES, 2008, 14 (02) :195-200
[8]   Most Escherichia coli strains overproducing chromosomal AmpC β-lactamase belong to phylogenetic group A [J].
Corvec, Stephane ;
Prodhomme, Adele ;
Giraudeau, Cecile ;
Dauvergne, Sandie ;
Reynaud, Alain ;
Caroff, Nathalie .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 60 (04) :872-876
[9]  
Farmer JJ, 2007, MANUAL OF CLINICAL MICROBIOLOGY, 9TH ED, P649
[10]   The AmpC phenotype in Norwegian clinical isolates of Escherichia coli is associated with an acquired ISEcp1-like ampC element or hyperproduction of the endogenous AmpC [J].
Haldorsen, Bjorg ;
Aasnaes, Bettina ;
Dahl, Kristin H. ;
Hanssen, Anne-Merethe ;
Simonsen, Gunnar S. ;
Walsh, Timothy R. ;
Sundsfjord, Arnfinn ;
Lundblad, Eirik W. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2008, 62 (04) :694-702