A microarray platform for parallel detection of five transgenic events in foods: A combined polymerase chain reaction - ligation detection reaction - universal array method

被引：34

作者：

Bordoni, R ^{[1
]}

Germini, A ^{[1
]}

Mezzelani, A ^{[1
]}

Marchelli, R ^{[1
]}

De Bellis, G ^{[1
]}

机构：

[1] CNR, Ist Tecnol Biomed, I-20090 Segrate, MI, Italy

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2005年 / 53卷 / 04期

关键词：

genetically modified organism (GMO); ligation detection reaction (LDR); microarray; universal array; multiplex; polymerase chain reaction (PCR);

D O I：

10.1021/jf0486949

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

We recently developed a multiplex polymerase chain reaction (PCR) system for the simultaneous detection of four transgenic maize (MON810, Bt176, Bt11, and GA21), one transgenic soybean (Roundup Ready), and two control genes (lectin and zein). Because PCR can lead to ambiguous interpretations due to low specificity, we have developed the ligation detection reaction (LDR) combined with a universal array as a molecular tool to confirm results of PCR analysis. Here, we describe the PCR-LDR-universal array procedure and demonstrate its specificity in revealing the presence of transgenic DNA in experimental samples, raw materials, and commercial foodstuffs.

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收藏

页码：912 / 918

页数：7

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