Secretoneurin and chemoattractant receptor interactions

被引：40

作者：

Kong, C

Gill, BM

Rahimpour, R

Xu, L

Feldman, RD

Xiao, Q

McDonald, TJ

Taupenot, L

Mahata, SK

Singh, B

O'Connor, DT

Kelvin, DJ

机构：

[1] Robarts Res Inst, Lab Mol Inflammat & Immunol, London, ON N6G 2V4, Canada

[2] Univ Western Ontario, Dept Microbiol & Immunol, London, ON, Canada

[3] Univ Western Ontario, Dept Med, London, ON, Canada

[4] Univ Calif San Diego, Dept Med, San Diego, CA 92161 USA

[5] Univ Calif San Diego, Ctr Mol Genet, San Diego, CA 92161 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1998年 / 88卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

chemotaxis; neuropeptide; G-protein coupled receptor;

D O I：

10.1016/S0165-5728(98)00094-0

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Secretoneurin (SN) is a 33-amino acid peptide derived from secretogranin II (chromogranin C) which induces chemotaxis of monocytes but not neutrophils. In this study, we found that SN interacted with specific cell surface binding sites on human monocytes. The chemoattractants MCP-1, MCP-2 or fMLP could not compete for SN binding sites suggesting SN may bind to a novel chemotactic receptor. Additional studies showed that neither SN nor MCP-2 induced a rise in cytosolic Ca2+, and chemotaxis to SN was inhibited by cholera toxin (CT) and pertussis toxin (PT). Chemotactic desensitization studies demonstrated that fMLP, MCP-1, SN, and MCP-2 could all desensitize monocytes to subsequent SN stimulation. Our results indicate that SN binds to a cell surface receptor expressed on monocytes and activates signaling pathways which are sensitive to CT and PT. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：91 / 98

页数：8