Simple and large-scale production of N-acetylneuraminic acid from N-acetyl-D-glucosamine and pyruvate using N-acyl-D-glucosamine 2-epimerase and N-acetylneuraminate lyase

被引：77

作者：

Maru, I ^{[1
]}

Ohnishi, J ^{[1
]}

Ohta, Y ^{[1
]}

Tsukada, Y ^{[1
]}

机构：

[1] Marukin Shoyu Co Ltd, Kyoto Res Labs, Uji, Kyoto 611, Japan

来源：

CARBOHYDRATE RESEARCH | 1998年 / 306卷 / 04期

关键词：

N-acetyl-D-glucosamine; N-acetyl-D-mannosamine; N-acetylneuraminate lyase; N-acyl-D-glucosamine; 2-epimerase; sialic acid;

D O I：

10.1016/S0008-6215(97)10106-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Acetylneuraminate lyase and N-acyl-D-glucosamine 2-epimerase had been cloned and overexpressed in Escherichia coli. Simultaneous use of these two enzymes and feeding of appropriate amounts of pyruvate to the reaction mixture made possible the high conversion of N-acetylneuraminic acid (Neu5Ac) from N-acetyl-D-glucosamine (GlcNAc) with a 77% conversion rate on a molar basis. As a result, 29 kg of Neu5Ac was obtained from 27 kg of GlcNAc. The product was recovered by direct crystallization, and verified as identical to authentic Neu5Ac. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：575 / 578

页数：4

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