Control of coleopteran insect pests through RNA interference

被引:1282
作者
Baum, James A.
Bogaert, Thierry
Clinton, William
Heck, Gregory R.
Feldmann, Pascale
Ilagan, Oliver
Johnson, Scott
Plaetinck, Geert
Munyikwa, Tichafa
Pleau, Michael
Vaughn, Ty
Roberts, James
机构
[1] Monsanto Co, Chesterfield, MO 63017 USA
[2] Devgen NV, B-9052 Ghent, Belgium
关键词
D O I
10.1038/nbt1359
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Commercial biotechnology solutions for controlling lepidopteran and coleopteran insect pests on crops depend on the expression of Bacillus thuringiensis insecticidal proteins(1,2), most of which permeabilize the membranes of gut epithelial cells of susceptible insects(3). However, insect control strategies involving a different mode of action would be valuable for managing the emergence of insect resistance. Toward this end, we demonstrate that ingestion of doublestranded (ds)RNAs supplied in an artificial diet triggers RNA interference in several coleopteran species, most notably the western corn rootworm (WCR) Diabrotica virgifera virgifera LeConte. This may result in larval stunting and mortality. Transgenic corn plants engineered to express WCR dsRNAs show a significant reduction in WCR feeding damage in a growth chamber assay, suggesting that the RNAi pathway can be exploited to control insect pests via in planta expression of a dsRNA.
引用
收藏
页码:1322 / 1326
页数:5
相关论文
共 25 条
[1]   Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana [J].
Allen, E ;
Xie, ZX ;
Gustafson, AM ;
Sung, GH ;
Spatafora, JW ;
Carrington, JC .
NATURE GENETICS, 2004, 36 (12) :1282-1290
[2]  
ANDERSON S, 2007, Patent No. 0050860
[3]  
[Anonymous], MOL CLONING
[4]  
ARMSTRONG CL, 2003, Patent No. 6603061
[5]   RNA silencing in plants [J].
Baulcombe, D .
NATURE, 2004, 431 (7006) :356-363
[6]   Parental RNAi in Tribolium (Coleoptera) [J].
Bucher, G ;
Scholten, J ;
Klingler, M .
CURRENT BIOLOGY, 2002, 12 (03) :R85-R86
[7]  
DREES BM, 1999, HDB CORN INSECTS
[8]   Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].
Fire, A ;
Xu, SQ ;
Montgomery, MK ;
Kostas, SA ;
Driver, SE ;
Mello, CC .
NATURE, 1998, 391 (6669) :806-811
[9]   RNA interference [J].
Hannon, GJ .
NATURE, 2002, 418 (6894) :244-251
[10]  
James C, 2003, CURR SCI INDIA, V84, P303