Reusable impedimetric aptasensor

被引:234
作者
Radi, AE [1 ]
Sánchez, JLA
Baldrich, E
O'Sullivan, CK
机构
[1] Mansoura Univ, Fac Sci, Dept Chem, Dumyat 34517, Egypt
[2] Univ Rovira & Virgili, Dept Chem Engn, Nanobiotechnol & Bioanal Grp, Tarragona, Spain
[3] Inst Catalana Recerca & Estudis Avancats, Barcelona 08010, Spain
关键词
D O I
10.1021/ac0505775
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN)(6)(3-/4-), using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by electrochemical impedance spectroscopy or cyclic voltammetry. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2.0 M NaCl solution, and the immobilized aptamer subsequently was used for repeated detection of thrombin. The aptamer-functionalized electrode showed a linear response of the charge-transfer resistance to the increase of thrombin concentration in the range of 5.0-35.0 nM and the thrombin was easily detectable to a concentration of 2.0 nM.
引用
收藏
页码:6320 / 6323
页数:4
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