Binding of human minichromosome maintenance proteins with histone H3

被引:113
作者
Ishimi, Y [1 ]
Ichinose, S [1 ]
Omori, A [1 ]
Sato, K [1 ]
Kimura, H [1 ]
机构
[1] HOKKAIDO UNIV,RES CTR MOL GENET,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN
关键词
D O I
10.1074/jbc.271.39.24115
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Minichromosome maintenance (MCM) proteins play essential roles in eukaryotic DNA replication, but their biochemical properties remain to be determined. We detected in HeLa cell extracts six proteins, CDC47, CDC46/MCM5, Cdc21, P1/MCM3, Mis5, and BM28/MCM2, by their binding to a specific antibody and by partial sequencing. The human homologs of the MCM2 (BM28), Mis5, Cdc21, and CDC47 proteins were tightly bound to a histone-Sepharose column and purified to near homogeneity, whereas the P1/MCM3 and CDC46/MCM5 proteins passed through. Among the four core histones, the human BM28/MCM2, Mis5, Cdc21, and CDC47 proteins had high affinity for histone H3. Immunoprecipitation with anti-Cdc21 antibody revealed that these four MCM proteins form complexes. These results are consistent with the findings that MCM proteins bind with chromatin in vivo.
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页码:24115 / 24122
页数:8
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