Structure-based enzyme engineering and its impact on in vitro glycorandomization

被引：73

作者：

Thorson, JS

Barton, WA

Hoffmeister, D

Albermann, C

Nikolov, DB

机构：

[1] Univ Wisconsin, Sch Pharm, Pharmaceut Sci Div, Lab Biosynthet Chem, Madison, WI 53705 USA

[2] Mem Sloan Kettering Canc Ctr, Cellular Biochem & Biophys Program, New York, NY 10021 USA

来源：

CHEMBIOCHEM | 2004年 / 5卷 / 01期

关键词：

antibiotics; enzyme catalysis; glycosyl phosphate; natural products; transferases;

D O I：

10.1002/cbic.200300620

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vitro glycorandomization (IVG) strategy. IVG is a versatile strategy with great potential for the generation of novel therapeutics as illustrated by the preparation of a library of diversified vancomycin analogues.

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收藏

页码：16 / 25

页数：10

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